2019-09-25
New High-Throughput Method for Elastin-like Polymer (ELP) Coacervate Analysis
彈(dan)性蛋(dan)白聚合物（ELP）凝(ning)聚層分析的新型(xing)高(gao)通量方法
Posted by Michelle Devoe
Michelle Devoe發表
December 2018 — A recent study by researchers from the University of New England and University of New Hampshire has demonstrated that flow imaging microscopy is an accurate, more efficient, and more informative method of elastin-like polymer (ELP) coacervate analysis than standard methods. ELP coacervates are a class of molecules with promising applications in drug delivery vehicles, tissue engineering, environmental remediation, and more. ELP coacervate architecture is stimuli-responsive and highly tunable, making them ideal for the above-mentioned applications.
2018年12月 - 新(xin)英格蘭大(da)學和新(xin)罕*爾(er)大(da)學的(de)研(yan)究(jiu)人(ren)員近的(de)一(yi)(yi)項研(yan)究(jiu)表(biao)明，與標準方法(fa)相比，流(liu)動(dong)成像顯微(wei)鏡是一(yi)(yi)種準確，更有(you)效，信息更豐富的(de)彈性(xing)蛋白(bai)樣聚合物(wu)（ELP）凝聚分析方法(fa)。 ELP凝聚層在作為藥物(wu)遞送的(de)載體，組(zu)織工程，環境修復等方面具有(you)廣闊的(de)應用(yong)前景。 ELP凝聚層結(jie)構具有(you)刺激響應性(xing)和高度可(ke)調性(xing)，使其成為上述應用(yong)的(de)理(li)想選擇。
ELP coacervates imaged by the FlowCam. Size and circularity, two of the 40+ properties that can be measured by the FlowCam, is used to sort coacervates. Image from Marvin et al. (2018).
ELP凝(ning)聚了由FlowCam成(cheng)像的圖像。 尺寸和圓度是FlowCam可(ke)以測量的40多個屬(shu)性中的兩個，用于(yu)對凝(ning)聚層進行分類。 圖片(pian)來自(zi)Marvin等。（2018）。
Standard methods for ELP coacervate analysis are indirect and cumbersome. Data from Visible-UV spectrophotometer turbidity measurements and dynamic light scattering (DLS) size measurements are superimposed to evaluate the formation of micron-scale aggregates, and particle geometry is constructed indirectly from diffusion data. Imaging analysis methods such as optical, electron, and scanning probe microscopy are useful methods to acquire size and shape data, however it is time-consuming and laborious to acquire a large enough sample size to get statistically significant data.
ELP凝(ning)聚(ju)層(ceng)分(fen)析的(de)(de)標準(zhun)方(fang)法(fa)是間接和(he)(he)麻煩的(de)(de)。 來自可見 - 紫外分(fen)光(guang)(guang)光(guang)(guang)度計濁度測量和(he)(he)動態光(guang)(guang)散(san)射(she)（DLS）尺(chi)寸測量的(de)(de)數據(ju)(ju)(ju)(ju)被疊加(jia)以評估微米級(ji)聚(ju)集(ji)體(ti)的(de)(de)形成(cheng)，并(bing)且顆粒幾何形狀間接地由擴散(san)數據(ju)(ju)(ju)(ju)構建。 諸如(ru)光(guang)(guang)學，電子和(he)(he)掃描探針顯(xian)微鏡(jing)之(zhi)類(lei)的(de)(de)成(cheng)像分(fen)析方(fang)法(fa)是獲取(qu)尺(chi)寸和(he)(he)形狀數據(ju)(ju)(ju)(ju)的(de)(de)有用方(fang)法(fa)，然而獲得(de)足夠大的(de)(de)樣本大小以獲得(de)統(tong)計上有效的(de)(de)數據(ju)(ju)(ju)(ju)是耗時且費(fei)力的(de)(de)。
In this study by the University of New England and University of New Hampshire, the size, morphology, and behavior of ELP coacervates subjected to various solvent conditions were measured and observed using the FlowCam particle analyzer. Results were validated by comparison with DLS and atomic force microscopy analyses. Flow imaging microscopy was demonstrated to be a successful method for ELP coacervate analysis. Additionally, flow imaging microscopy reported additional findings that were not measured using the DLS, microscopy, or Visible-UV spectrophotometry.
在新英格(ge)蘭(lan)大學(xue)和新罕*爾大學(xue)的(de)(de)這(zhe)項研究中，使用(yong)FlowCam顆粒分(fen)析儀測量和觀察(cha)經(jing)受各(ge)種(zhong)溶(rong)劑條件的(de)(de)ELP凝(ning)聚層的(de)(de)尺寸，形態和行為。 通過與DLS和原子力顯(xian)微(wei)鏡分(fen)析的(de)(de)比較驗證結果。流(liu)動成像(xiang)顯(xian)微(wei)鏡被證明是(shi)ELP凝(ning)聚層分(fen)析的(de)(de)成功方法。 此外，流(liu)動成像(xiang)顯(xian)微(wei)鏡提供了DLS，顯(xian)微(wei)鏡或可見紫外分(fen)光光度法不能獲得(de)的(de)(de)結果。
在線客服